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排序方式: 共有442条查询结果,搜索用时 31 毫秒
431.
432.
Kengo Hamamura Naoya Matsunaga Eriko Ikeda Hideaki Kondo Hisako Ikeyama Kazutaka Tokushige Kazufumi Itcho Yoko Furuichi Yuya Yoshida Masaki Matsuda Kaori Yasuda Atsushi Doi Yoshifumi Yokota Toshiaki Amamoto Hironori Aramaki Yasuhiro Irino Satoru Koyanagi Shigehiro Ohdo 《The Journal of biological chemistry》2016,291(10):4913-4927
Chronic kidney disease (CKD) is associated with an increase in serum retinol; however, the underlying mechanisms of this disorder are poorly characterized. Here, we found that the alteration of hepatic metabolism induced the accumulation of serum retinol in 5/6 nephrectomy (5/6Nx) mice. The liver is the major organ responsible for retinol metabolism; accordingly, microarray analysis revealed that the hepatic expression of most CYP genes was changed in 5/6Nx mice. In addition, D-box-binding protein (DBP), which controls the expression of several CYP genes, was significantly decreased in these mice. Cyp3a11 and Cyp26a1, encoding key proteins in retinol metabolism, showed the greatest decrease in expression in 5/6Nx mice, a process mediated by the decreased expression of DBP. Furthermore, an increase of plasma transforming growth factor-β1 (TGF-β1) in 5/6Nx mice led to the decreased expression of the Dbp gene. Consistent with these findings, the alterations of retinol metabolism and renal dysfunction in 5/6Nx mice were ameliorated by administration of an anti-TGF-β1 antibody. We also show that the accumulation of serum retinol induced renal apoptosis in 5/6Nx mice fed a normal diet, whereas renal dysfunction was reduced in mice fed a retinol-free diet. These findings indicate that constitutive Dbp expression plays an important role in mediating hepatic dysfunction under CKD. Thus, the aggravation of renal dysfunction in patients with CKD might be prevented by a recovery of hepatic function, potentially through therapies targeting DBP and retinol. 相似文献
433.
Junya Azuma Ronald J. Wong Takeshi Morisawa Mark Hsu Lars Maegdefessel Hui Zhao Flora Kalish Yosuke Kayama Matthew B. Wallenstein Alicia C. Deng Joshua M. Spin David K. Stevenson Ronald L. Dalman Philip S. Tsao 《PloS one》2016,11(2)
Heme oxygenase-1 (HO-1), the rate-limiting enzyme in heme degradation, is a cytoprotective enzyme upregulated in the vasculature by increased flow and inflammatory stimuli. Human genetic data suggest that a diminished HO-1 expression may predispose one to abdominal aortic aneurysm (AAA) development. In addition, heme is known to strongly induce HO-1 expression. Utilizing the porcine pancreatic elastase (PPE) model of AAA induction in HO-1 heterozygous (HO-1+/-, HO-1 Het) mice, we found that a deficiency in HO-1 leads to augmented AAA development. Peritoneal macrophages from HO-1+/- mice showed increased gene expression of pro-inflammatory cytokines, including MCP-1, TNF-alpha, IL-1-beta, and IL-6, but decreased expression of anti-inflammatory cytokines IL-10 and TGF-beta. Furthermore, treatment with heme returned AAA progression in HO-1 Het mice to a wild-type profile. Using a second murine AAA model (Ang II-ApoE-/-), we showed that low doses of the HMG-CoA reductase inhibitor rosuvastatin can induce HO-1 expression in aortic tissue and suppress AAA progression in the absence of lipid lowering. Our results support those studies that suggest that pleiotropic statin effects might be beneficial in AAA, possibly through the upregulation of HO-1. Specific targeted therapies designed to induce HO-1 could become an adjunctive therapeutic strategy for the prevention of AAA disease. 相似文献
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Immunizing potencies of vaccines prepared from various strains of Salmonella were graded by comparing the mortality rate of immunized mice after challenge with highly virulent strains of either Salmonella enteritidis or S. typhimurium. The resistance against this challenge infection was shown to be conferred by joint immunization with a specific factor, which was represented by O specific lipopolysaccharide of smooth strains, and cross-protection factor, which was a major potent factor in live vaccine. The distribution of this cross-protection factor in rough mutants of S. typhimurium was found to be limited to strains which possessed a polysaccharide chain longer than that of glucose1-less mutant. The potency conferring cross-resistance was found to be maintained partly in formalin-killed cells and cell walls of the strains harboring cross-protection factor but not in lipopolysaccharide extracted from such strains. 相似文献
437.
Takehito Nakazawa Iori Yamaguchi Yufan Zhang Chinami Saka Hongli Wu Keita Kayama Moriyuki Kawauchi Masahiro Sakamoto Yoichi Honda 《Environmental microbiology》2023,25(10):1909-1924
Lignin-modifying enzymes (LMEs), which include laccases (Lacs), manganese peroxidases (MnPs), versatile peroxidases (VPs), and lignin peroxidases (LiPs), have been considered key factors in lignin degradation by white-rot fungi because they oxidize lignin model compounds and depolymerize synthetic lignin in vitro. However, it remains unclear whether these enzymes are essential/important in the actual degradation of natural lignin in plant cell walls. To address this long-standing issue, we examined the lignin-degrading abilities of multiple mnp/vp/lac mutants of Pleurotus ostreatus. One vp2/vp3/mnp3/mnp6 quadruple-gene mutant was generated from a monokaryotic wild-type strain PC9 using plasmid-based CRISPR/Cas9. Also, two vp2/vp3/mnp2/mnp3/mnp6, two vp2/vp3/mnp3/mnp6/lac2 quintuple-gene mutants, and two vp2/vp3/mnp2/mnp3/mnp6/lac2 sextuple-gene mutants were generated. The lignin-degrading abilities of the sextuple and vp2/vp3/mnp2/mnp3/mnp6 quintuple-gene mutants on the Beech wood sawdust medium reduced drastically, but not so much for those of the vp2/vp3/mnp3/mnp6/lac2 mutants and the quadruple mutant strain. The sextuple-gene mutants also barely degraded lignin in Japanese Cedar wood sawdust and milled rice straw. Thus, this study presented evidence that the LMEs, especially MnPs and VPs, play a crucial role in the degradation of natural lignin by P. ostreatus for the first time. 相似文献
438.
Hisako Nishihara Sc. D. Leila J. Walker M. S. Joseph W. Landau M. D. Victor D. Newcomer M.D. 《Mycopathologia》1967,32(4):291-296
Summary The changes in the serum glycoproteins and the development of specific antibodies were examined during the course of experimental coccidioidomycosis in rats. The total glycoprotein, seromucoid hexose, seromucoid protein, and haptoglobin levels were signigicantly higher in sera from infected animals compared with sera from non-infected controls. These changes were evident at three days but not at two weeks following inoculation withC. immitis. The non-seromucoid hexose and total protein concentrations were not significantly different between infected animals and controls. The highest percentages of animals exhibiting positive tests for complement fixing and precipitin antibodies and positive cultures forC. immitis in organs at autopsy were found one week after infection.This study was supported in part by USPHS Grants T1 A1 52-07, A106048-02 and the Dermatologic Research Foundation of California, Inc. 相似文献
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The germination and tube growth of Camellia japonica pollen were stopped on a myrmicacincontaining (50–200 ppm) culture medium, but were restored again when the pollen grains were transferred to a myrmicacin-free medium. Myrmicacin inhibits the movement of Golgi vesicles utilized for the formation of the callose layer in the pollen grain before the germination, and the growing tube tip wall. 相似文献